Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-7-23
pubmed:abstractText
Introduction of apurinic sites into phi X174 am3 DNA leads to loss of biological activity when measured in a transfection assay. For single-stranded DNA, approximately one apurinic site constitutes a lethal hit; for double-stranded (RFI) DNA, approximately 3.5 hits per strand are lethal. When the reversion frequency of am3 DNA is measured, no increase due to depurination is observed above the background level. However, a large increase in reversion frequency is observed when the same DNA is assayed by using spheroplasts derived from bacteria previously exposed to UV light. The results suggest that apurinic sites are impediments to a replicating DNA polymerase; however, nucleotides can be incorporated opposite these sites under SOS-induced conditions. We estimate the frequency of mutagenesis per apurinic site to be less than 1 in 1400 in normal spheroplasts and 1 in 100 in SOS-induced spheroplasts.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-1069305, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-1260750, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-14097355, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-203936, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-226880, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-266709, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-333435, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-373805, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-375225, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-375236, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-376517, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-392601, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-398538, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4136142, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4151811, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-430573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4360077, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-450070, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4559796, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4590108, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4601435, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4626532, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4706005, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-4797, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-5044802, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-5437664, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-593377, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-6448843, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-6930660, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-731693, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-786147, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-795416, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-799253, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-922734, http://linkedlifedata.com/resource/pubmed/commentcorrection/6453349-95052
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1773-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Depurination causes mutations in SOS-induced cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.