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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1981-7-23
|
| pubmed:abstractText |
Fragments from section 3 of the salivary gland X chromosome of D. melanogaster were dissected with a micromanipulator. The DNA was extracted, cut and ligated to a lambda vector in a volume of a few nanoliters in an oil chamber monitored through a microscope. From about 10 pg of DNA we obtained 80 recombinant clones, a sample of which were analysed and shown to contain Drosophila DNA which hybridises in situ to the region of section 3 of the X chromosome. With this technique we can isolate clones from any desired region as small as 200 kb from the euchromatic arms of polytene chromosomes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0009-5915
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
82
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
205-16
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6452995-Bacteriophage lambda,
pubmed-meshheading:6452995-Chromosomes,
pubmed-meshheading:6452995-Cloning, Molecular,
pubmed-meshheading:6452995-Drosophila melanogaster,
pubmed-meshheading:6452995-History, Modern 1601-,
pubmed-meshheading:6452995-Micromanipulation,
pubmed-meshheading:6452995-Nucleic Acid Hybridization,
pubmed-meshheading:6452995-Salivary Glands
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes.
|
| pubmed:publicationType |
Journal Article,
Historical Article,
Research Support, Non-U.S. Gov't
|