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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1981-4-13
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pubmed:abstractText |
Fluorescein isothiocyanate is a highly specific inhibitor of the Ca2+-ATPase from sarcoplasmic reticulum. The Ca2+ pumping is inhibited completely at a fluorescein isothiocyanate concentration half that of the ATPase protein, indicating that the protein is at least a dimer. ATP protected specifically against fluorescein isothiocyanate inhibition, indicating that fluorescein isothiocyanate may react at the nucleotide binding site of the ATPase (probably with a reactive lysine residue). The fluorescein is incorporated almost exclusively into the 105 kdalton catalytic polypeptide of the ATPase and digestion by trypsin gives rise to a fluorescein-labelled 45 kdalton fragment. Conformational changes induced by addition of Ca can be studied conveniently with the fluorescein-labelled ATPase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
626
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
255-61
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:6450619-Animals,
pubmed-meshheading:6450619-Binding Sites,
pubmed-meshheading:6450619-Biological Transport, Active,
pubmed-meshheading:6450619-Calcium,
pubmed-meshheading:6450619-Calcium-Transporting ATPases,
pubmed-meshheading:6450619-Fluorescein-5-isothiocyanate,
pubmed-meshheading:6450619-Fluoresceins,
pubmed-meshheading:6450619-Kinetics,
pubmed-meshheading:6450619-Macromolecular Substances,
pubmed-meshheading:6450619-Molecular Weight,
pubmed-meshheading:6450619-Peptide Fragments,
pubmed-meshheading:6450619-Protein Binding,
pubmed-meshheading:6450619-Sarcoplasmic Reticulum,
pubmed-meshheading:6450619-Spectrophotometry,
pubmed-meshheading:6450619-Thiocyanates,
pubmed-meshheading:6450619-Trypsin
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pubmed:year |
1980
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pubmed:articleTitle |
Indications for an oligomeric structure and for conformational changes in sarcoplasmic reticulum Ca2+-ATPase labelled selectively with fluorescein.
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pubmed:publicationType |
Journal Article
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