Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1981-2-24
|
pubmed:abstractText |
As a first step in the development of a multiple-marker, mammalian cell mutagenesis assay system, we have isolated a Chinese hamster ovary (CHO) cell line that is heterozygous for both the adenine phosphoribosyltransferase (aprt) and thymidine kinase (tk) loci. Presumptive aprt+/- heterozygotes with intermediate levels of APRT activity were selected from unmutagenized CHO cell populations on the basis of resistance to low concentrations of the adenine analog, 8-azaadenine. a functional aprt+/ heterozygote with approximately 50% wild-type APRT activity was subsequently used to derive sublines that were also heterozygous for the tk locus. Biochemical and genetic characterization of one such subline, CHO-AT3-2, indicated that it was indeed heterozygous at both the aprt and tk loci. CHO-AT3-2 cells permitted single-step selection of mutants resistant for 8-azaadenine or 5-fluorodeoxyuridine, allowing quantitation and direct comparison of mutation induction at the autosomal aprt or tk loci, as well as in the gene involved in ouabain resistance or at the X-linked, hypoxanthine--guanine phosphoribosyltransferase (hgprt) locus. Significant dose-dependent increases in mutation frequency were observed for all 4 genetic markers after treatment of CHO-AT3-2 cells with ethyl methanesulfonate.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-azaadenine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenine Phosphoribosyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Ethyl Methanesulfonate,
http://linkedlifedata.com/resource/pubmed/chemical/Floxuridine,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxanthine...,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Pentosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Kinase
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0027-5107
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
72
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
187-205
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:6449663-Adenine,
pubmed-meshheading:6449663-Adenine Phosphoribosyltransferase,
pubmed-meshheading:6449663-Animals,
pubmed-meshheading:6449663-Cricetinae,
pubmed-meshheading:6449663-Cricetulus,
pubmed-meshheading:6449663-Drug Resistance,
pubmed-meshheading:6449663-Ethyl Methanesulfonate,
pubmed-meshheading:6449663-Female,
pubmed-meshheading:6449663-Floxuridine,
pubmed-meshheading:6449663-Hybrid Cells,
pubmed-meshheading:6449663-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:6449663-Mutagenicity Tests,
pubmed-meshheading:6449663-Ouabain,
pubmed-meshheading:6449663-Pentosyltransferases,
pubmed-meshheading:6449663-Thymidine Kinase
|
pubmed:year |
1980
|
pubmed:articleTitle |
Mutagenicity testing in mammalian cells. I. Derivation of a Chinese hamster ovary cell line heterozygous for the adenine phosphoribosyltransferase and thymidine kinase loci.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|