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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1985-3-8
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pubmed:abstractText |
Protocols for detecting picogram quantities of aflatoxin M1 in dairy products were established. Milk samples were subjected to a reverse phase Sep-Pak C18 cartridge treatment before analysis by an enzyme-linked immunosorbent assay (ELISA) according to previously published procedures. M1 in yogurt, brick cheddar, and ripened Brie cheese was extracted by a modified Pons method, subjected to a normal phase silica cartridge treatment, and analyzed by ELISA. The detection limits for M1 in milk, yogurt, cheddar, and Brie were 10, 10, 50, and 25 ppt (ng/kg), respectively. Recovery for M1 added to these products was in the range 70-110%. Good agreement was found for M1 levels in several naturally contaminated milk samples analyzed by both ELISA and liquid chromatography.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0004-5756
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1098-101
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6440890-Aflatoxin M1,
pubmed-meshheading:6440890-Aflatoxins,
pubmed-meshheading:6440890-Animals,
pubmed-meshheading:6440890-Cattle,
pubmed-meshheading:6440890-Cheese,
pubmed-meshheading:6440890-Dairy Products,
pubmed-meshheading:6440890-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6440890-Food Microbiology,
pubmed-meshheading:6440890-Milk,
pubmed-meshheading:6440890-Yogurt
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pubmed:articleTitle |
Direct enzyme-linked immunosorbent assay for determining aflatoxin M1 at picogram levels in dairy products.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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