Linked Life Data

6440414

Source:http://linkedlifedata.com/resource/pubmed/id/6440414

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-2-21
pubmed:abstractText
Sera and sputa of 12 cystic fibrosis patients suffering from chronic Pseudomonas aeruginosa lung infections were assayed for immune complexes using the Raji cell assay. Whereas all sera were negative, 33% of the sputa were positive for immune complexes. Sera and sputa of these patients were also assayed for antibodies against P.aeruginosa alkaline protease (AP) and elastase (Ela) and sputa for AP and Ela, using radioimmunoassays. All patients revealed antibody titers to the proteases in serum (1:5-1:1000) and eight patients had antibody titers to the proteases in sputum (1:5-1:100); all sputa were negative for AP and Ela. Sputum immune complexes and IgG were separated from whole sputum on a Protein A-Sepharose C1-4B column and treated with 0.75 M 2-mercaptoethanol and iodoacetamide after elution. After treatment, 58% of the sputa were positive for AP and/or Ela (5-200 ng/ml sputum). The present study shows that proteases of P.aeruginosa are bound in immune complexes after the initiation of the immune response to these antigens and it yields new insights into the role of these proteases in chronic lung infection in cystic fibrosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0108-0202
pubmed:author
pubmed:issnType
Print
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
307-12
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Detection of proteases of Pseudomonas aeruginosa in immune complexes isolated from sputum of cystic fibrosis patients.
pubmed:publicationType
Journal Article