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rdf:type |
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lifeskim:mentions |
umls-concept:C0001443,
umls-concept:C0008266,
umls-concept:C0015155,
umls-concept:C0025723,
umls-concept:C0035553,
umls-concept:C0205103,
umls-concept:C0205117,
umls-concept:C0443286,
umls-concept:C0728938,
umls-concept:C1306673,
umls-concept:C1517945,
umls-concept:C1705535
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pubmed:issue |
23
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pubmed:dateCreated |
1985-1-25
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pubmed:abstractText |
Bacterial, cytoplasmic and organellar ribosomes from a wide phylogenetic spectrum of organisms have a characteristic m6(2)Am6(2)A structure near the 3' end of the RNA of the small ribosomal subunit (SSU). We have studied one of the few exceptions to this extremely conserved post-transcriptionally modified sequence, i.e. dimethylation of only one of the two A's in chloroplasts from Euglena gracilis. It was established that only the A closest to the 5' end is dimethylated, the other one being unmodified. The methylation reaction was studied in vitro using ribosomes from a kasugamycin resistant mutant (ksgA) of Escherichia coli and purified methyl-transferase. Using limited amounts of the methyl donor S-adenosylmethionine (SAM) a partial level of methylation (50% of control) was attained. It is shown that in this case the 3' proximal A is dimethylated while the other is not. This suggests that dimethylation takes place in two successive stages. Apparently in E. gracilis chloroplasts the first stage of methylation does not occur.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-1102710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-383710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-383712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-409999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-4329247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6178009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6253497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6294602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6343825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6363901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6575236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6709501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6728686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6778393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-6809748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-7010312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6440121-793864
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9205-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:6440121-Adenine,
pubmed-meshheading:6440121-Adenosine,
pubmed-meshheading:6440121-Biological Evolution,
pubmed-meshheading:6440121-Chloroplasts,
pubmed-meshheading:6440121-Cloacin,
pubmed-meshheading:6440121-Escherichia coli,
pubmed-meshheading:6440121-Euglena gracilis,
pubmed-meshheading:6440121-Methylation,
pubmed-meshheading:6440121-Methyltransferases,
pubmed-meshheading:6440121-RNA, Ribosomal,
pubmed-meshheading:6440121-RNA Processing, Post-Transcriptional,
pubmed-meshheading:6440121-Ribosomes
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pubmed:year |
1984
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pubmed:articleTitle |
Partial methylation of two adjacent adenosines in ribosomes from Euglena gracilis chloroplasts suggests evolutionary loss of an intermediate stage in the methyl-transfer reaction.
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pubmed:publicationType |
Journal Article
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