| pubmed-article:6439744 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6439744 | lifeskim:mentions | umls-concept:C0599779 | lld:lifeskim |
| pubmed-article:6439744 | lifeskim:mentions | umls-concept:C0012739 | lld:lifeskim |
| pubmed-article:6439744 | lifeskim:mentions | umls-concept:C0015498 | lld:lifeskim |
| pubmed-article:6439744 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:6439744 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:6439744 | pubmed:dateCreated | 1985-1-31 | lld:pubmed |
| pubmed-article:6439744 | pubmed:abstractText | An experimental animal model of disseminated intravascular coagulation (DIC) induced by the co-infusion of coagulant-active phospholipid and activated Factor X (Factor Xa) is described. The infusion of Factor Xa at a dose of 6.6 X 10(-12) mol/kg with phosphatidylcholine/phosphatidylserine (PCPS) lipid vesicles at a dose of 4.0 X 10(-8) mol/kg was associated with significant falls in the levels of fibrinogen and Factors V and VIII, and a bleeding diathesis developed. Assays of Factors V and VIII were performed by a one-stage prothrombin time and activated partial thrombin time system, respectively. In additional experiments, the effect of the same dose combination of Factor Xa/PCPS on Factor V kinetics was studied by preinfusing 125I-labeled Factor V. After Factor Xa/PCPS infusion, Factors VIII and V were reduced at 2 min by 90 and 50% of the preinfusion levels, respectively, and at 1 h by 80 and 75%, respectively. During the same period, there was little change in the total circulating radioactivity. Autoradiography indicated small but detectable levels of circulating proteolytic products of Factor V that comigrated with peptides obtained by the incubation of Factor V with Factor Xa and activated protein C. The majority of radioactivity remained associated with the intact single-chain precursor Factor V. These observations suggested maintenance of the precursor pool after the onset of DIC. This was confirmed by performing two-stage assays of Factors V and VIII, whereby each was completely converted to the active cofactor, i.e., Va and VIII:Ca, by preincubation of the test sample with thrombin before assaying in a one-stage system as before. The Factor V levels assayed by the two-stage procedure did not change appreciably over 1 h. The Factor VIII levels fell but corrected within 1 h at a time when the level measured by a one-stage assay remained depressed. These results indicate that in the dog, infusion of Factor Xa/PCPS induces changes characteristic of DIC, and this is associated with the appearance of Factor V peptides characteristic of the expression of Factor Xa and activated protein C-like activities. The differences noted between the one-stage and two-stage assays suggest that the one-stage assay is measuring the activated fraction of each cofactor and not the total level of the available precursor for each activated species. The results suggest a close correlation between the activated fraction of both cofactors and the hemostatic abnormality that occurs in DIC. | lld:pubmed |
| pubmed-article:6439744 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6439744 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6439744 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6439744 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:6439744 | pubmed:issn | 0021-9738 | lld:pubmed |
| pubmed-article:6439744 | pubmed:author | pubmed-author:MannK GKG | lld:pubmed |
| pubmed-article:6439744 | pubmed:author | pubmed-author:NesheimM EME | lld:pubmed |
| pubmed-article:6439744 | pubmed:author | pubmed-author:GilesA RAR | lld:pubmed |
| pubmed-article:6439744 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6439744 | pubmed:volume | 74 | lld:pubmed |
| pubmed-article:6439744 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6439744 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6439744 | pubmed:pagination | 2219-25 | lld:pubmed |
| pubmed-article:6439744 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:meshHeading | pubmed-meshheading:6439744-... | lld:pubmed |
| pubmed-article:6439744 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6439744 | pubmed:articleTitle | Studies of Factors V and VIII:C in an animal model of disseminated intravascular coagulation. | lld:pubmed |
| pubmed-article:6439744 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6439744 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6439744 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6439744 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6439744 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6439744 | lld:pubmed |
