Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1985-1-31
pubmed:abstractText
An experimental animal model of disseminated intravascular coagulation (DIC) induced by the co-infusion of coagulant-active phospholipid and activated Factor X (Factor Xa) is described. The infusion of Factor Xa at a dose of 6.6 X 10(-12) mol/kg with phosphatidylcholine/phosphatidylserine (PCPS) lipid vesicles at a dose of 4.0 X 10(-8) mol/kg was associated with significant falls in the levels of fibrinogen and Factors V and VIII, and a bleeding diathesis developed. Assays of Factors V and VIII were performed by a one-stage prothrombin time and activated partial thrombin time system, respectively. In additional experiments, the effect of the same dose combination of Factor Xa/PCPS on Factor V kinetics was studied by preinfusing 125I-labeled Factor V. After Factor Xa/PCPS infusion, Factors VIII and V were reduced at 2 min by 90 and 50% of the preinfusion levels, respectively, and at 1 h by 80 and 75%, respectively. During the same period, there was little change in the total circulating radioactivity. Autoradiography indicated small but detectable levels of circulating proteolytic products of Factor V that comigrated with peptides obtained by the incubation of Factor V with Factor Xa and activated protein C. The majority of radioactivity remained associated with the intact single-chain precursor Factor V. These observations suggested maintenance of the precursor pool after the onset of DIC. This was confirmed by performing two-stage assays of Factors V and VIII, whereby each was completely converted to the active cofactor, i.e., Va and VIII:Ca, by preincubation of the test sample with thrombin before assaying in a one-stage system as before. The Factor V levels assayed by the two-stage procedure did not change appreciably over 1 h. The Factor VIII levels fell but corrected within 1 h at a time when the level measured by a one-stage assay remained depressed. These results indicate that in the dog, infusion of Factor Xa/PCPS induces changes characteristic of DIC, and this is associated with the appearance of Factor V peptides characteristic of the expression of Factor Xa and activated protein C-like activities. The differences noted between the one-stage and two-stage assays suggest that the one-stage assay is measuring the activated fraction of each cofactor and not the total level of the available precursor for each activated species. The results suggest a close correlation between the activated fraction of both cofactors and the hemostatic abnormality that occurs in DIC.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-1154319, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-1237299, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-1238394, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-12845, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-13434757, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-334327, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-4433081, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-4733239, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-4796071, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-500617, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-508770, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-5450638, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-588557, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-6177716, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-6294139, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-6783711, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-6809076, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-6848522, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-6895378, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-7034817, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-7043191, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-762076, http://linkedlifedata.com/resource/pubmed/commentcorrection/6439744-854877
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2219-25
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Studies of Factors V and VIII:C in an animal model of disseminated intravascular coagulation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't