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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1978-6-17
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pubmed:abstractText |
Transcription of the human adenovirus 5 genome in transformed rat embryo cells (DFK3) was investigated using two different approaches. Preferential digestion of transcribed viral sequences by DNase I was analysed using kinetics of renaturation of 32P-labeled Ad5 HpaI restriction fragments in the presence of material which was stable after nuclease treatment. The second approach was the hybridization of 32P-labeled nuclear RNA from transformed cells with Ad5 restriction fragments which were attached to a nitrocellulose filter. These two methods gave similar results. It was found that not all integrated regions of the Ad5 genome are active in transformed cells. 2,5 copies of the HpaI-E fragment of Ad5 DNA were found in transformed DFK3 cell line. Nuclear RNA from these cells hybridized to HpaI-E fragment of Ad5 DNA, but only about half of sequences of the integrated HpaI-E fragment was sensitive to DNase I digestion.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-1057474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-1099215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-169095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-169096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-184963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-185395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-4354250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-4473553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-4734785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-4898584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-837446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-948749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-950687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/643600-986101
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:643600-Adenoviridae,
pubmed-meshheading:643600-Base Sequence,
pubmed-meshheading:643600-Cell Line,
pubmed-meshheading:643600-Cell Transformation, Viral,
pubmed-meshheading:643600-DNA, Viral,
pubmed-meshheading:643600-DNA Restriction Enzymes,
pubmed-meshheading:643600-Genes, Viral,
pubmed-meshheading:643600-RNA, Viral,
pubmed-meshheading:643600-Transcription, Genetic
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pubmed:year |
1978
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pubmed:articleTitle |
Studies of the transcription of viral genome in adenovirus 5 transformed cells.
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pubmed:publicationType |
Journal Article
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