Linked Life Data

6431565

Source:http://linkedlifedata.com/resource/pubmed/id/6431565

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-8-31
pubmed:abstractText
When human polymorphonuclear leukocytes (PMNL) were incubated with exogenous 14C-arachidonic acid only traces of 5-lipoxygenase products including 5-HETE and leukotriene B4 were detected. On the other hand, considerable amounts of cyclo-oxygenase metabolites including PGE2, TXB2, HHT, PGD2, PGF2 alpha and 6-keto-PGF1 alpha as well as mono-HETE's migrating in the vicinity of 12-HETE were formed. Coincubation of PMNL with platelets did not lead to any detectable activation of the 5-lipoxygenase pathway. 10 microM U-60,257, an inhibitor of leukotriene synthesis, had no effect on the low basal 5-lipoxygenase activity nor on other lipoxygenases in either PMNL or during coincubation of PMNL with platelets. The formation of cyclo-oxygenase products was neither affected by U-60,257. It also failed to have any effect on the aggregation of PMNL induced by arachidonic acid, leukotriene B4 or platelet activating factor. The present study indicates that U-60,257, which has been reported to be a specific inhibitor of leukotriene synthesis, has no effect on the cyclo-oxygenase or on lipoxygenases other than the 5-lipoxygenase (i.e. 12- and possibly 15-) in human PMNL and obviously neither in human platelets.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0034-5164
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
355-65
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
U-60,257 has no effect on the metabolism of arachidonic acid in nonstimulated human polymorphonuclear leukocytes.
pubmed:publicationType
Journal Article