Linked Life Data

6431282

Source:http://linkedlifedata.com/resource/pubmed/id/6431282

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1984-9-12
pubmed:abstractText
The surface proteins of six stages of Trypanosoma cruzi were labeled by Iodogen-catalized surface iodination and analyzed by one and two dimensional polyacrylamide gel electrophoresis. These stages included bloodstream trypomastigotes, culture-form trypomastigotes, amastigotes, staphylomastigotes, epimastigotes, and metacyclic trypomastigotes. Antigens recognized by serum antibodies were detected by Western blotting against serum from mice hyperimmunized against bloodstream trypomastigotes. Bloodstream trypomastigotes, culture-form trypomastigotes and staphylomastigotes contained several surface proteins of molecular weight (Mr) 90 000 and isoelectric points (pI) between 5.0 and 7.5. Western blotting reveals that at least two proteins of 90 000 Mr represent the major antigens seen on bloodstream trypomastigotes, culture-form trypomastigotes, staphylomastigotes and amastigotes. However, a 90 000 Mr protein was not detected by either Western blotting or surface iodination on epimastigotes or metacyclic trypomastigotes. The major surface proteins on these latter two stages were represented by several 72 000 Mr proteins with pI values between 5.2 and 5.8. An interesting result of this survey is that a 90 000 Mr surface antigen was present on staphylomastigotes, a stage which can be grown in cell free medium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Major surface proteins and antigens on the different in vivo and in vitro forms of Trypanosoma cruzi.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.