Linked Life Data

6431036

Source:http://linkedlifedata.com/resource/pubmed/id/6431036

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1984-9-14
pubmed:abstractText
The production of 5-S-cysteinyldopa by a newly established melanoma cell line GAK is reported. The cell line was derived from a metastatic inguinal lymph node of vulvar malignant melanoma. The cell line grew well without interruption for over 4 years, GAK cells were proved to have melanin granules and tyrosinase activity in their cytoplasma by Masson's staining and dopa reaction, respectively. Melanin granules were ultrastructually identified as melanosomes in various maturing stages. The chromosomal number varied widely and showed aneuploidly, but the modal chromosomal number was stable in the hypotriploid range. GAK cells were transplanted to nude mice and produced tumors resembling the original. Because glucose-6-phosphate dehydrogenase of GAK revealed a type B (slow) mobility pattern on electrophoresis, the possibility of Hela cell contamination could be completely excluded. High performance liquid chromatography revealed "5-S-cysteinyldopa", a new tumor marker of malignant melanoma, in culture media of GAK cells. The cell line described may serve as a representative model system for basic and clinical studies on malignant melanoma.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0300-9165
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
883-92
pubmed:dateRevised
2011-7-29
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
[A newly established melanoma cell line (GAK) with 5-S-cysteinyldopa phenotype].
pubmed:publicationType
Journal Article, English Abstract