| pubmed-article:6430685 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6430685 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:6430685 | lifeskim:mentions | umls-concept:C0018207 | lld:lifeskim |
| pubmed-article:6430685 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:6430685 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:6430685 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6430685 | pubmed:dateCreated | 1984-9-20 | lld:pubmed |
| pubmed-article:6430685 | pubmed:abstractText | The role of somatomedin-C (Sm-C) in the acquisition of granulosa cell progesterone biosynthesis was investigated in vitro in a primary culture of rat granulosa cells cultured for 72 h under serum-free conditions. Basal progesterone accumulation was negligible and remained unaffected by treatment with highly purified Sm-C (50 ng/ml). Whereas treatment with FSH (20 ng/ml) produced a 9-fold increase in progesterone accumulation, the concurrent application of increasing concentrations (0.3-50 ng/ml) of Sm-C brought about dose-dependent increments in the FSH-stimulated accumulation of progesterone with a median effective dose of 4.0 +/- (SE) 0.3 ng/ml and a maximal response 9.6-fold greater than that induced by FSH alone. A monoclonal antibody raised against Sm-C (sm 1.2) produced complete immunoneutralization of the synergistic interaction between FSH and Sm-C, supporting the specificity of the Sm-C effect and arguing against the possible involvement of copurified contaminant(s) in the preparation used. Treatment of granulosa cells with the highest dose of Sm-C tested (50 ng/ml), in the absence or presence of FSH, did not result in significant alterations in cell number, DNA content, plating efficiency or viability. Taken together, our findings indicate that Sm-C is capable of synergizing with FSH in the induction of granulosa cell progesterone biosynthesis. Significantly, this ability of Sm-C to augment differentiated phenotypic expression of the developing granulosa cell is distinct from its well established growth-promoting property and may thus represent a novel biologic effect of this polypeptide. | lld:pubmed |
| pubmed-article:6430685 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6430685 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:6430685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6430685 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6430685 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:6430685 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:6430685 | pubmed:author | pubmed-author:Van WykJ JJJ | lld:pubmed |
| pubmed-article:6430685 | pubmed:author | pubmed-author:AdashiE YEY | lld:pubmed |
| pubmed-article:6430685 | pubmed:author | pubmed-author:ResnickC ECE | lld:pubmed |
| pubmed-article:6430685 | pubmed:author | pubmed-author:SvobodaM EME | lld:pubmed |
| pubmed-article:6430685 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6430685 | pubmed:volume | 115 | lld:pubmed |
| pubmed-article:6430685 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6430685 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6430685 | pubmed:pagination | 1227-9 | lld:pubmed |
| pubmed-article:6430685 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:meshHeading | pubmed-meshheading:6430685-... | lld:pubmed |
| pubmed-article:6430685 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6430685 | pubmed:articleTitle | A novel role for somatomedin-C in the cytodifferentiation of the ovarian granulosa cell. | lld:pubmed |
| pubmed-article:6430685 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6430685 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6430685 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6430685 | lld:pubmed |
