Linked Life Data

6430034

Source:http://linkedlifedata.com/resource/pubmed/id/6430034

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-8-7
pubmed:abstractText
The effect of glibenclamide on the osmotic resistance of beta-cells was measured using isolated beta-cells from ob/ob-mice. The beta-cells were incubated at different osmolarity and the diameters of the approximately spherical beta-cells were measured at 22 degrees C or at 37 degrees C with the aid of a screw micrometer eyepiece fitted to a light microscope. A near linear decrease of beta-cell diameter was found with increasing osmolarity (111-617 mosm/l). Control experiments showed that the membrane stabilizers, imipramine (0.1 mmol/l) or tetracaine (1 mmol/l), strongly reduced the osmotic swelling induced by low osmolarity (180 mosm/l). Glibenclamide (0.001 or 0.2 mmol/l) did not affect the beta-cell diameter at normal osmolarity (317 mosm/l) but reduced the swelling induced by hypoosmolarity (180 mosm/l) and the shrinking induced by hyperosmolarity (617 mosm/l). It is suggested that glibenclamide increases the osmotic resistance of isolated beta-cells by changing transmembrane flow of ions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0001-6772
pubmed:author
pubmed:issnType
Print
pubmed:volume
120
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
407-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Effect of glibenclamide on the osmotic resistance of pancreatic beta-cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't