|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1984-6-8
|
|
pubmed:abstractText |
Type IV collagen could not be extracted from human placenta using 6M-urea containing 10mM-dithiothreitol, indicating that the type IV molecule is stabilized within the basement membrane by covalent cross-links. However, various forms of type IV collagen molecule were extractable by means of increasingly severe proteolytic conditions. Type IV collagen tetramers ('spiders') lacking only the C-terminal globular region (NC1) were further digested to the 'long-form' 7S fragment and an assortment of helical rod-like molecules derived from the 'leg' region. These molecules were separated by salt fractionation and examined by rotary-shadowing electron microscopy. Isolation of these fractions from placenta which had been reduced with NaB3H4 revealed that both the 7S (N-terminal) and C-terminal regions contained significant proportions of reducible lysine-derived cross-links. These cross-links were shown to be exclusively the stable oxo-imine hydroxylysino-5-oxonorleucine. The number of the cross-links per molecule was significantly lower than might be expected in order to fully stabilize the molecule. These results suggest that the keto-imine cross-links in type IV collagen have been stabilized in part by conversion into an unknown non-reducible form, although a sensitive immunoassay failed to show the presence of any pyridinoline. Comparison with the fibrous collagen from placenta suggested that the rate of this conversion is much greater in basement-membrane collagen.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-371988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-456357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-4704059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-4722452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-5577447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6157354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6217835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6249253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6262125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6274634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6286238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6626172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6626173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6762208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6772152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-6853554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-7073734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-7271798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-728134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-7356471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-7458897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-7470049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-761615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6426460-901463
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
218
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
713-23
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6426460-Basement Membrane,
pubmed-meshheading:6426460-Borohydrides,
pubmed-meshheading:6426460-Chromatography, Ion Exchange,
pubmed-meshheading:6426460-Collagen,
pubmed-meshheading:6426460-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6426460-Female,
pubmed-meshheading:6426460-Humans,
pubmed-meshheading:6426460-Microscopy, Electron,
pubmed-meshheading:6426460-Models, Molecular,
pubmed-meshheading:6426460-Pepsin A,
pubmed-meshheading:6426460-Peptide Fragments,
pubmed-meshheading:6426460-Placenta,
pubmed-meshheading:6426460-Pregnancy,
pubmed-meshheading:6426460-Protein Conformation,
pubmed-meshheading:6426460-Time Factors
|
|
pubmed:year |
1984
|
|
pubmed:articleTitle |
Cross-linking in type IV collagen.
|
|
pubmed:publicationType |
Journal Article
|
