Linked Life Data

6423006

Source:http://linkedlifedata.com/resource/pubmed/id/6423006

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-5-10
pubmed:abstractText
Acetone precipitates of bovine follicular fluid from small (3-5 mm) and large (10-20 mm) follicles were fractionated by nondissociative procedures involving chromatography on Sephacryl S-300, DEAE cellulose, and Sepharose CL-2B. Most of the proteoglycan material eluted with a relatively low molecular weight (Kav = approximately 0.6) from Sepharose CL-2B. Comparisons of the proteoglycans from small versus large follicles showed respective differences of 20 versus 34% protein, 74 versus 48% chondroitin sulfate-B, and 4 versus 8% other sugars. The protein moiety of the proteoglycan from small follicles exhibited greater proportions of aspartic acid, serine and glycine, but lower proportions of theonine, alanine and valine, than its analog from large follicles. Chromatography on Sepharose CL-2B in 4 M guanidine-HCl failed to alter the elution position of the proteoglycans. However, noticeable decreases occurred in the protein and total amino acid content of the proteoglycans, especially from large follicles, suggesting preparations obtained from the nondissociative procedures contained aggregates of peptides, glycosaminoglycans, and/or proteoglycan fragments. Proteoglycans from both sizes of follicles were enriched in serine, glutamic acid and glycine, but diminished in leucine and lysine following the dissociative gel filtration. Differences in composition of the proteoglycans may be related to follicular differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
397-409
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Characteristics of proteoglycans isolated from small and large bovine ovarian follicles.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't