Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1984-4-24
|
| pubmed:abstractText |
An enzyme-linked immunosorbent assay (ELISA) was developed for detecting Brucella abortus. Carbodiimide cyanamide was used as an antigen-coupling agent and NH4Cl was used to neutralize residual active charges present on the cuvette surface. Suitable ELISA reactions were observed in vaginal swabs placed in phosphate-buffered saline solution. The ELISA described may be useful under controlled laboratory conditions for detecting B abortus-infected cows shedding bacteria in vaginal secretions.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0002-9645
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
45
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
32-4
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6422806-Animals,
pubmed-meshheading:6422806-Antigens, Bacterial,
pubmed-meshheading:6422806-Brucella Vaccine,
pubmed-meshheading:6422806-Brucella abortus,
pubmed-meshheading:6422806-Brucellosis, Bovine,
pubmed-meshheading:6422806-Cattle,
pubmed-meshheading:6422806-Cattle Diseases,
pubmed-meshheading:6422806-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6422806-Female,
pubmed-meshheading:6422806-Leukorrhea,
pubmed-meshheading:6422806-Vaccination,
pubmed-meshheading:6422806-Vagina
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Application of an enzyme-linked immunosorbent assay for detection of Brucella antigens in vaginal discharge of cows.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
|