Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-3-16
pubmed:abstractText
Gonococci, grown on agar, frequently give rise to opaque colonies. This opacity phenotype is associated with the presence of one or more outer membrane proteins of approximately 28,000 mol weight. These proteins are included within a class of proteins named proteins II. A method is described to isolate and purify the opacity-associated proteins from Neisseria gonorrhoeae. This method uses high concentrations of calcium and a zwitterionic detergent at pH 4.0. Under these conditions proteins II are readily solubilized from the outer membrane. Further purification is achieved by ion exchange and molecular sieve chromatography in the presence of the zwitterionic detergent. The opacity-associated proteins are very basic with isoelectric points varying between 9.0 to 10.0. Further evidence for their basic nature is their behavior on ion exchange chromatography and their amino acid composition.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-101459, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-1070002, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-14047217, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-178649, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-337309, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-403181, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-4129205, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-415006, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-415007, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-4979098, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-56416, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6029938, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6102108, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6406636, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6769811, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6769820, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6790441, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6804388, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-6990986, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-7012112, http://linkedlifedata.com/resource/pubmed/commentcorrection/6420502-89171
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
452-62
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Purification and partial characterization of the opacity-associated proteins of Neisseria gonorrhoeae.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't