Linked Life Data

6418820

Source:http://linkedlifedata.com/resource/pubmed/id/6418820

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-2-22
pubmed:abstractText
This investigation describes a new procedure for the preparation of immunogenic liposomes which circumvents the need to synthesize the N-(hapten)-substituted derivatives of phosphatidylethanolamine that were previously employed for this purpose. The method is based on the generation of liposomes containing the N-hydroxysuccinimide (NHS) esters of either palmitic acid, cholesteryl-hemisuccinate, or N-succinyl-phosphatidylethanolamine. Reaction of these preformed liposomes with a hapten that possesses a substitutable amino group (e.g., DNP-lysine) results in covalent attachment of the hapten to the lipid bilayers. As a consequence of this binding, the liposomes can elicit formation of hapten-specific plaque-forming cells in mice. The reliability of this procedure is indicated by the fact that these liposomes share the essential immunological properties of liposomes sensitized by incorporation of N-substituted phosphatidylethanolamine derivatives (e.g., DNP-Cap-PE). Thus, the magnitude of the response was found to be dependent on: (a) the presence of lipid A in the liposomes; (b) the phospholipid composition of the liposomes; (c) the distance separating the DNP determinant from the liposomal surface. Additional applications of liposomes, which contain the NHS esters, are indicated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-306
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
An alternative procedure for the preparation of immunogenic liposomal model membranes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't