Linked Life Data

6418808

Source:http://linkedlifedata.com/resource/pubmed/id/6418808

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-2-23
pubmed:abstractText
The lactate dehydrogenase (LDH) isoenzyme pattern (expressed as the B:A subunit ratio) and two enzymes of the purine metabolism adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) (expressed as the ADA/PNP ratio) were studied in human prenatal thymocytes, in subsets of human infant thymocytes, and in peripheral T lymphocytes. Prothymocytes were enriched by E rosette depletion, cortical thymocytes were enriched with a monoclonal antibody rosette technique using OKT6, and medullary thymocytes were enriched either with a monoclonal antibody rosette technique using OKT3 or with complement-mediated cytolysis using normal fresh rabbit serum. Peripheral T lymphocytes were isolated from normal adult peripheral blood by E rosette sedimentation. Prenatal thymocytes had the lowest B:A ratio. In the infant thymuses, prothymocytes had a lower B:A ratio (0.99 +/- 0.10) than the cortical thymocytes (1.04 +/- 0.08). The medullary thymocytes obtained either by OKT3 selection or normal rabbit serum cytotoxic treatment had higher B:A ratios (1.30 +/- 0.15 and 1.42 +/- 0.17, respectively). The highest B:A ratio is found in peripheral T lymphocytes (2.07 +/- 0.28) together with the lowest ADA/PNP ratio (0.50 +/- 0.07). The B:A ratios are paralleled by a progressive decrease in the ADA/PNP ratio. These findings indicate that during intrathymic T cell development, important changes occur not only in the activities of the enzymes of the purine metabolism but also in the distribution of the LDH isoenzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
730-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
LDH analysis of human thymocytes and thymocyte subsets.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't