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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1983-12-21
|
| pubmed:abstractText |
The effects of low molecular weight (LMW) protease inhibitors of microbial origin were evaluated on the intracellular degradation of beta-galactosidase purified from Aspergillus oryzae and taken up by cultured human skin fibroblasts with beta-galactosidase deficiency. Only thiol protease inhibitors showed an effect to increase the enzyme activity. E-64, a specific inhibitor of thiol proteases, prolonged 3-fold a half life of the exogenous beta-galactosidase and when the enzyme was supplied as liposomes, the half life was prolonged 9-fold in these cells. The role of thiol proteases in the degradation of enzyme molecules was discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0014-4827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
148
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
525-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6414834-Biological Transport,
pubmed-meshheading:6414834-Cells, Cultured,
pubmed-meshheading:6414834-Fibroblasts,
pubmed-meshheading:6414834-Galactosidases,
pubmed-meshheading:6414834-Gangliosidoses,
pubmed-meshheading:6414834-Half-Life,
pubmed-meshheading:6414834-Humans,
pubmed-meshheading:6414834-Kinetics,
pubmed-meshheading:6414834-Protease Inhibitors,
pubmed-meshheading:6414834-Skin,
pubmed-meshheading:6414834-beta-Galactosidase
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Effects of thiol protease inhibitors on intracellular degradation of exogenous beta-galactosidase in cultured human skin fibroblasts.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|