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pubmed-article:6413248pubmed:abstractTextThe primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the beta chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of Mr 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the alpha chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by alpha and beta chain regions, respectively.lld:pubmed
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pubmed-article:6413248pubmed:articleTitleBiosynthesis of rabbit haptoglobin: chemical evidence for a single chain precursor.lld:pubmed
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