Linked Life Data

6409813

Source:http://linkedlifedata.com/resource/pubmed/id/6409813

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-9-9
pubmed:abstractText
To determine whether a non-A, non-B hepatitis agent contained essential lipids, we extracted with chloroform a dilution of human plasma that contained approximately 10(4) chimpanzee infectious doses of non-A, non-B hepatitis virus and then tested for infectivity in chimpanzees. In addition, we treated a serum containing hepatitis B virus in the same way. Both of these samples were also sham extracted as controls. Known chloroform-sensitive and chloroform-resistant viruses were added directly to the hepatitis-containing serum or plasma as internal controls or to fetal calf serum as external controls and were assayed for infectivity in vitro after chloroform extraction or sham extraction. All infectivity of the diluted plasma that contained at least 10(4) chimpanzee infective doses of non-A, non-B hepatitis agent and all infectivity of the serum that contained 10(3.5) chimpanzee infective doses of hepatitis B virus were destroyed by chloroform. The chloroform-sensitive control viruses were completely inactivated, but the chloroform-resistant control viruses lost less than 0.5 log10 of infectivity. Sham-extracted non-A, non-B hepatitis agent-containing plasma was shown to maintain its infectivity in chimpanzees that had initially been inoculated with the chloroform-extracted plasma. Thus, both hepatitis type B and non-A, non-B hepatitis appear to be caused by viruses that can be inactivated by a lipid solvent.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-1185011, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-163436, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-195505, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-4136143, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-451589, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-5360526, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-632054, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-6780630, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-6783707, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-6797865, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-6799586, http://linkedlifedata.com/resource/pubmed/commentcorrection/6409813-76017
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
816-21
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Inactivation of hepatitis B virus and non-A, non-B hepatitis by chloroform.
pubmed:publicationType
Journal Article, Comparative Study