Linked Life Data

6409561

Source:http://linkedlifedata.com/resource/pubmed/id/6409561

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-9-23
pubmed:abstractText
Immunosuppressive activity of alpha-globulin fraction of human blood plasma (Cohn fraction IV) was studied to determine the possible clinical utilization of this fraction as such, purified, or as the starting material for isolation of the active factor. The ethanol fractions IV, isolated and waste in the production of blood derivatives were found to be directly inapplicable because of relatively low activity and poor solubility. Therefore purification experiments were done to remove the denatured proteins and lipids and to increase the specific activity, they were performed on laboratory scale using DEAE ionex chromatography, and on semipilot scale utilizing the principles of ethanol fractionation of plasma. From the viewpoint of technology, the latter procedure seems more feasible; partial purification yielded good solubility and somewhat higher specific activity. Under study are further techniques capable of eliminating the contaminating proteins with do not carry the immunosuppressively active component, as well as the effectivity of pasteurization at 60 degrees C/10 hrs., which seems indispensable with respect to inactivation of infectious hepatitis viruses and the potential clinical application. Experimental isolations of the active component have led immunosuppressively active low-molecular part, which, however, is chemically nonhomogeneous. Its subsequent subfractionation will be necessary.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0139-9179
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-15
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Study on immunosuppressive activity of alpha-globulin fraction of human blood plasma.
pubmed:publicationType
Journal Article