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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1983-8-17
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pubmed:abstractText |
We have developed a specific radioimmunoassay to quantify NADPH: cytochrome P-450 reductase. The assay is based on the use of 125I-labelled NADPH: cytochrome P-450 reductase as the radiolabelled antigen and can detect quantities of this protein in amounts as low as 30 pg. The results of the radioimmunoassay demonstrates that the 2.7-fold increase in enzyme activity in rat liver microsomal membranes after phenobarbital treatment is due to increased amounts of the protein. beta-Naphthoflavone treatment, however, did not alter the activity or the quantity of this enzyme in microsomes. The quantification of NADPH: cytochrome P-450 reductase in the microsomes isolated from control and phenobarbital- and beta-naphthoflavone-treated animals permits the calculation of the ratio of this protein to that of total cytochromes P-450. A molar ratio of 15:1 (cytochromes P-450/NADPH: cytochrome P-450 reductase) was calculated for control and phenobarbital-treated animals. This ratio increased to 21:1 after beta-naphthoflavone treatment. Thus the molar ratio of these proteins in liver microsomes can vary with exposure of the animals to particular xenobiotics.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-107862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-14209972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-203576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-390562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-399809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4153601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4153862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-416020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4168680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4181161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4288784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4366168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4378769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4383307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4395592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-6161126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-6186523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-6768748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-6769479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-6781411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-6802825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-7152025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-7317020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6409093-826521
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
211
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
333-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6409093-Animals,
pubmed-meshheading:6409093-Benzoflavones,
pubmed-meshheading:6409093-Immune Sera,
pubmed-meshheading:6409093-Immunodiffusion,
pubmed-meshheading:6409093-Intracellular Membranes,
pubmed-meshheading:6409093-Male,
pubmed-meshheading:6409093-Microsomes, Liver,
pubmed-meshheading:6409093-NADPH-Ferrihemoprotein Reductase,
pubmed-meshheading:6409093-Phenobarbital,
pubmed-meshheading:6409093-Radioimmunoassay,
pubmed-meshheading:6409093-Rats,
pubmed-meshheading:6409093-Rats, Inbred Strains,
pubmed-meshheading:6409093-beta-Naphthoflavone
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pubmed:year |
1983
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pubmed:articleTitle |
Quantification of NADPH: cytochrome P-450 reductase in liver microsomes by a specific radioimmunoassay technique.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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