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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1983-8-26
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pubmed:abstractText |
Ninety-five porcine pancreases were incubated at temperatures of 0, 20, or 37 degrees C for periods of 1-32 h. After incubation at 37 degrees C for 2-4 h, islet cells retain their morphologic and functional integrity, whereas acinar cells become necrotic. Because of acinar destruction, the pancreas also becomes soft and amenable to mechanical separation with a simple new device. Temperature-controlled incubation therefore may be a useful first step in the isolation of islet cells from large animal pancreases.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0160-564X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
250-3
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:6409061-Animals,
pubmed-meshheading:6409061-Cell Separation,
pubmed-meshheading:6409061-Incubators,
pubmed-meshheading:6409061-Insulin,
pubmed-meshheading:6409061-Islets of Langerhans,
pubmed-meshheading:6409061-Islets of Langerhans Transplantation,
pubmed-meshheading:6409061-Pancreas,
pubmed-meshheading:6409061-Swine
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pubmed:year |
1983
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pubmed:articleTitle |
Whole-organ incubation as a first step in the isolation of pancreatic islets from large animals.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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