pubmed-article:6401709 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0033809 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0019053 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0814810 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0031669 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
pubmed-article:6401709 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
pubmed-article:6401709 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:6401709 | pubmed:dateCreated | 1983-3-17 | lld:pubmed |
pubmed-article:6401709 | pubmed:abstractText | A hemolysin determinant was cloned from Pseudomonas aeruginosa PA103 by inserting Sau3a-generated DNA fragments between the BamHI sites of the lambda replacement vector WL47.1. A 9.5-kilobase HindIII fragment encoding the hemolysin was subcloned from this phage and inserted into the plasmid vector pHC79 to generate the recombinant plasmid pKC95. Escherichia coli K-12 strains harboring pKC95 exhibited zones of hemolysis after several days of growth on blood agar plates. Hemolysis was shown to be due to phospholipase C activity by using the chromogenic substrate p-nitrophenylphosphorylcholine. Deletion mutants of pKC95 were isolated, and polypeptides expressed from these plasmids were examined by using the E. coli minicell system. A polypeptide of 78,000 daltons was associated with phospholipase C activity. The hemolytic activity was cell associated when expressed in E. coli. | lld:pubmed |
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pubmed-article:6401709 | pubmed:language | eng | lld:pubmed |
pubmed-article:6401709 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:6401709 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:6401709 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:6401709 | pubmed:month | Feb | lld:pubmed |
pubmed-article:6401709 | pubmed:issn | 0021-9193 | lld:pubmed |
pubmed-article:6401709 | pubmed:author | pubmed-author:ArbuthnottJ... | lld:pubmed |
pubmed-article:6401709 | pubmed:author | pubmed-author:DouganGG | lld:pubmed |
pubmed-article:6401709 | pubmed:author | pubmed-author:ColemanKK | lld:pubmed |
pubmed-article:6401709 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:6401709 | pubmed:volume | 153 | lld:pubmed |
pubmed-article:6401709 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:6401709 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:6401709 | pubmed:pagination | 909-15 | lld:pubmed |
pubmed-article:6401709 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:6401709 | pubmed:year | 1983 | lld:pubmed |
pubmed-article:6401709 | pubmed:articleTitle | Cloning, and expression in Escherichia coli K-12, of the chromosomal hemolysin (phospholipase C) determinant of Pseudomonas aeruginosa. | lld:pubmed |
pubmed-article:6401709 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:6401709 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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