Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-3-17
pubmed:abstractText
A hemolysin determinant was cloned from Pseudomonas aeruginosa PA103 by inserting Sau3a-generated DNA fragments between the BamHI sites of the lambda replacement vector WL47.1. A 9.5-kilobase HindIII fragment encoding the hemolysin was subcloned from this phage and inserted into the plasmid vector pHC79 to generate the recombinant plasmid pKC95. Escherichia coli K-12 strains harboring pKC95 exhibited zones of hemolysis after several days of growth on blood agar plates. Hemolysis was shown to be due to phospholipase C activity by using the chromogenic substrate p-nitrophenylphosphorylcholine. Deletion mutants of pKC95 were isolated, and polypeptides expressed from these plasmids were examined by using the E. coli minicell system. A polypeptide of 78,000 daltons was associated with phospholipase C activity. The hemolytic activity was cell associated when expressed in E. coli.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-114487, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-13697414, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-13762792, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-183567, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-319344, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-323852, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-327263, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-363523, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-378395, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-387722, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-392234, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-405620, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-4370620, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-4959184, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-6100899, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-6254843, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-6260575, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-7007421, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-7031483, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-7039497, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-788919, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-809500, http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-829886
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
909-15
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Cloning, and expression in Escherichia coli K-12, of the chromosomal hemolysin (phospholipase C) determinant of Pseudomonas aeruginosa.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't