rdf:type |
|
lifeskim:mentions |
umls-concept:C0008633,
umls-concept:C0009015,
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0019053,
umls-concept:C0031669,
umls-concept:C0033809,
umls-concept:C0185117,
umls-concept:C0814810,
umls-concept:C1521761,
umls-concept:C2911684
|
pubmed:issue |
2
|
pubmed:dateCreated |
1983-3-17
|
pubmed:abstractText |
A hemolysin determinant was cloned from Pseudomonas aeruginosa PA103 by inserting Sau3a-generated DNA fragments between the BamHI sites of the lambda replacement vector WL47.1. A 9.5-kilobase HindIII fragment encoding the hemolysin was subcloned from this phage and inserted into the plasmid vector pHC79 to generate the recombinant plasmid pKC95. Escherichia coli K-12 strains harboring pKC95 exhibited zones of hemolysis after several days of growth on blood agar plates. Hemolysis was shown to be due to phospholipase C activity by using the chromogenic substrate p-nitrophenylphosphorylcholine. Deletion mutants of pKC95 were isolated, and polypeptides expressed from these plasmids were examined by using the E. coli minicell system. A polypeptide of 78,000 daltons was associated with phospholipase C activity. The hemolytic activity was cell associated when expressed in E. coli.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-114487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-13697414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-13762792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-183567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-319344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-323852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-327263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-363523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-378395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-387722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-392234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-405620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-4370620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-4959184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-6100899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-6254843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-6260575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-7007421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-7031483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-7039497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-788919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-809500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6401709-829886
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0021-9193
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
153
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
909-15
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:6401709-Cloning, Molecular,
pubmed-meshheading:6401709-Escherichia coli,
pubmed-meshheading:6401709-Genes, Bacterial,
pubmed-meshheading:6401709-Hemolysin Proteins,
pubmed-meshheading:6401709-Hemolysis,
pubmed-meshheading:6401709-Phospholipases,
pubmed-meshheading:6401709-Plasmids,
pubmed-meshheading:6401709-Pseudomonas aeruginosa,
pubmed-meshheading:6401709-Type C Phospholipases
|
pubmed:year |
1983
|
pubmed:articleTitle |
Cloning, and expression in Escherichia coli K-12, of the chromosomal hemolysin (phospholipase C) determinant of Pseudomonas aeruginosa.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|