Linked Life Data

6396301

Source:http://linkedlifedata.com/resource/pubmed/id/6396301

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-3-27
pubmed:abstractText
n-Octyl-beta-D-thioglucopyranoside (octylthioglucoside), a new nonionic detergent, was synthesized. Properties of this detergent and its applicability to membrane biochemistry were investigated. The critical micelle concentration of this detergent was determined to be 9 mM. Membrane proteins of Escherichia coli were effectively solubilized with octylthioglucoside at 25-35 mM. The solubilizing power was equivalent to that of n-octyl-beta-D-glucopyranoside (octylglucoside). Reconstitution of the melibiose carrier into liposomes was performed by the detergent dilution procedure. It was found that the concentration range of octylthioglucoside for successful reconstitution (45-70 mM) was considerably wider than that of octylglucoside (43-46 mM), thus giving more reproducible results. Octylthioglucoside is more stable than octylglucoside. Furthermore, the former is synthesized with high yield at low cost whereas the latter is very expensive. Thus, we conclude that octylthioglucose is superior to octylglucoside, and is very useful in membrane biochemistry.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1593-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Use of n-octyl-beta-D-thioglucoside, a new nonionic detergent, for solubilization and reconstitution of membrane proteins.
pubmed:publicationType
Journal Article