Linked Life Data

6394952

Source:http://linkedlifedata.com/resource/pubmed/id/6394952

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-2-27
pubmed:databankReference
pubmed:abstractText
As a first approach towards studying the regulation of hemolysin synthesis in Escherichia coli, we have fused lacZ into the four hly genes (hlyC, hlyA, hlyBa and hlyBb) using the Mud-1 (Mu::lacZ, Y, Apr) phage. The sites of insertion of Mud-1 within the various hly genes of the Hly plasmid pHly152 were determined by the hemolytic phenotype of the Hly- mutants (Hly-ex/Hly-in or Hly-ex/Hly+in) and by complementation of these Hly- mutants with recombinant plasmids carrying cloned hly genes. It was found that hlyC, hlyA and hlyBa are transcribed from a relatively weak promoter (hlypL) located in front of hlyC. The activity of beta-galactosidase is considerably lower when Mud-1 is integrated in hlyBa than when it is inserted in hlyC, suggesting a considerable decline in hly gene expression from hlyC to hlyBa. The DNA sequence upstream of the coding region of hlyC was found to promote galK gene expression when a fragment covering this region was inserted into the promoter-probe vector pKO-11. A putative promoter sequence, which could correspond to hlypL, was identified in this sequence. The hlyBb gene appears to be transcribed from a different promoter and the direction of transcription seems to be opposite to that of the hlyC, A, Ba operon. The strength of this promoter (hlypR), based on the level of beta-galactosidase activity of Mud-1 insertion mutants in hlyBb, is considerably higher than that of hlypL.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:volume
197
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
196-203
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Expression and regulation of the plasmid-encoded hemolysin determinant of Escherichia coli.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't