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pubmed:abstractText |
Lipid synthesis from [1-14C] acetate was stimulated by insulin in rat hepatocytes. Vanadate also enhanced lipid synthesis, although its effect was about 2-3 times higher than that of insulin, whereas ouabain significantly decreased hepatic lipid synthesis. The stimulatory effects of both insulin and vanadate were not dependent on extracellular Ca2+. Also, this stimulation was not mimicked by either ouabain or ouabain plus vanadate, even though they are expected to induce a non-specific increase of cytoplasmic Ca2+ concentration. These results suggest that insulin and vanadate stimulation of lipid synthesis may not be mediated by changes in Ca2+ fluxes through plasma membrane.
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