Linked Life Data

6392081

Source:http://linkedlifedata.com/resource/pubmed/id/6392081

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1985-2-21
pubmed:abstractText
The description of the Syrian hamster major histocompatibility complex (MHC), Hm-1, has progressed to the point that multiple class II alloantigens have been defined using structural and functional studies. However, no comparable success has been achieved using allotypic differences to detect class I molecules. We now report that xenoantisera raised in other species against hamster tissues have made it possible to describe class I MHC homologues in the hamster. Evidence which confirms that these molecules exist includes (1) on immunoprecipitation of radiolabeled lymphoid cell lysates, heterodimers of approximate molecular weight 47 000 and 12 000 are identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the latter representing hamster beta 2-microglobulin; (2) by direct immunoprecipitation these molecules are ubiquitously expressed on hamster tissues; (3) partial N-terminal amino acid sequence analysis reveals striking homology with class I molecules described in other species. In addition, the amino acid sequence data reveal that two class I molecules are expressed on the surfaces of hamster cells. On two-dimensional PAGE analysis, these molecules are invariant among the several strains of genetically disparate hamsters available for study. We conclude that (1) hamsters have the capacity to make class I MHC molecules, (2) at least two genetic loci are dedicated to this purpose, and (3) no allelic forms can be detected, suggesting that there is no class I polymorphism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0093-7711
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
603-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Syrian hamsters express two monomorphic class I major histocompatibility complex molecules.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.