GENERIC 6386986

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0041942, umls-concept:C0062756, umls-concept:C0150312
pubmed:issue	1
pubmed:dateCreated	1984-11-27
pubmed:abstractText	Urea inhibits the activity of histidinol dehydrogenase from Escherichia coli B, prevents precipitation of the enzyme by specific antibodies and dissolves immunoprecipitates which were formed in the absence of urea. However, immunoadsorption of the enzyme to Sepharose-bound antibodies can take place in the presence of high concentrations (5-8 M) of urea. The immobilized antibody-bound enzyme exhibits almost full activity after removal of the urea and is inhibited by soluble specific antibodies in the presence of urea. The possibility of using immunoadsorption in the presence of urea for the study of insoluble proteins is discussed.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/1305440
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Alcohol Oxidoreductases, http://linkedlifedata.com/resource/pubmed/chemical/Urea, http://linkedlifedata.com/resource/pubmed/chemical/histidinol dehydrogenase
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	0022-1759
pubmed:author	pubmed-author:AndornNN, pubmed-author:AronovitchJJ
pubmed:issnType	Print
pubmed:day	12
pubmed:volume	73
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	49-55
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:6386986-Alcohol Oxidoreductases, pubmed-meshheading:6386986-Escherichia coli, pubmed-meshheading:6386986-Immunosorbent Techniques, pubmed-meshheading:6386986-Urea
pubmed:year	1984
pubmed:articleTitle	Immunoadsorption of histidinol dehydrogenase in the presence of urea.
pubmed:publicationType	Journal Article