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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-11-19
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pubmed:abstractText |
The location of flurophores specifically bound to the lactose/H+ carrier of Escherichia coli was ascertained by the use of various collisional quenchers. The reporter groups were (1) the pyrenyl residue of N-(1-pyrenyl)maleimide attached to the essential cysteine residue 148, which is presumably at or near the galactoside binding site, and (2) the dansyl moieties of a series of fluorescent substrate molecules. The accessibility of these fluorophores from the lipid phase was assessed by nitroxyl-labelled fatty acids and phospholipids. By using a series of nitroxyl-labelled fatty acids carrying the quencher at different positions in the acyl chain, the position of a quenchable fluorophore with respect to the membrane normal can be determined. The accessibility of fluophores from the aqueous phase was assessed by using a water-soluble quencher, the N-methylpicolinium ion. The results of quenching studies suggest that the galactoside binding site is located within the carrier and that this binding site communicates with the aqueous phase through a pore.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Affinity Labels,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosides,
http://linkedlifedata.com/resource/pubmed/chemical/LacY protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/lactose permease
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
776
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-58
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6383472-Affinity Labels,
pubmed-meshheading:6383472-Binding Sites,
pubmed-meshheading:6383472-Escherichia coli,
pubmed-meshheading:6383472-Escherichia coli Proteins,
pubmed-meshheading:6383472-Fluorescent Dyes,
pubmed-meshheading:6383472-Galactosides,
pubmed-meshheading:6383472-Glycosides,
pubmed-meshheading:6383472-Kinetics,
pubmed-meshheading:6383472-Membrane Lipids,
pubmed-meshheading:6383472-Membrane Transport Proteins,
pubmed-meshheading:6383472-Monosaccharide Transport Proteins,
pubmed-meshheading:6383472-Spectrometry, Fluorescence,
pubmed-meshheading:6383472-Structure-Activity Relationship,
pubmed-meshheading:6383472-Symporters,
pubmed-meshheading:6383472-Water
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pubmed:year |
1984
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pubmed:articleTitle |
Localization of the galactoside binding site in the lactose carrier of Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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