Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-9-28
pubmed:abstractText
Electrophoretic analyses of the products of cell-free translation of elastin mRNA isolated from 17-day chick-embryo thoracic arteries have demonstrated that the elastin mRNA codes for polypeptides that are slightly larger than the cellular tropoelastin polypeptides synthesized and secreted by matrix-free artery cells. Pulse-chase experiments with cells labelled with [3H]proline established that newly synthesized tropoelastin polypeptides were associated solely with membrane-bound particulate fractions. Cell-free translation of membrane-bound and free polyribosomes isolated from artery cells revealed that the tropoelastin mRNA was associated predominantly with the membrane-bound fraction. When rough-microsomal fractions, isolated from cells labelled with [3H]proline for 10 min, were treated with proteinases in the presence and in the absence of detergent, the nascent tropoelastin polypeptides were shown to be susceptible to proteolysis only when the integrity of the membranes was destroyed by detergent treatment. In similar experiments tropoelastin polypeptides synthesized by membrane-bound polyribosomes in the nuclease-treated reticulocyte lysate were also resistant to the proteolytic-enzyme treatment. The results suggest that tropoelastin polypeptides are synthesized on membrane-bound polyribosomes and discharged into the lumen of the endoplasmic reticulum with co-translational removal of a signal peptide.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-1096303, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-1127879, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-176945, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-188639, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-218909, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-227370, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-4327463, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-4376955, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-4448420, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-449904, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-486120, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-5000644, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-518553, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-526264, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-597239, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-597256, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-6167404, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-6258540, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-6358099, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-6455244, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-6883163, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-7035466, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-7149737, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-7240184, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-8039, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-823012, http://linkedlifedata.com/resource/pubmed/commentcorrection/6383346-944578
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
221
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-400
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Elastin biosynthesis in chick-embryo arteries. Studies on the intracellular site of synthesis of tropoelastin.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't