Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1984-10-3
pubmed:abstractText
The psbA gene from higher plants, which codes for the atrazine herbicide binding protein of photosystem II (QB protein), has been recently sequenced by various laboratories. From these data there are two potential translation sites, one yielding a protein of 38,500 kd and another a protein of 34,500 kd. In the present study, cloned psbA gene sequences from maize, tobacco, and pea have been expressed in a highly defined E. coli in vitro transcription/translation system. In order to determine the start site of translation, we also have employed a simplified E. coli system designed to synthesize the first di- or tripeptide of the gene product. From these results, it is clear that the first ATG of the longest open reading frame of the psbA gene, that begins fMet-Thr, is not recognized in vitro. Instead, the next downstream Met at position 37 is the initiation site, since the expected dipeptide fMet-Ile is synthesized from all psbA clones. These data are in accord with the in vivo results that the gene product is a precursor protein of 34,500 kd.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-1093180, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-112101, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-1201011, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-160561, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-163927, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-16453439, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-16592541, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-16592577, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-16593262, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-17773338, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-374074, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-561072, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6261223, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6271631, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6289308, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6290998, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6314845, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6574909, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6760818, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-6944150, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-7027258, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-7106304, http://linkedlifedata.com/resource/pubmed/commentcorrection/6382165-7462238
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6221-30
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
In vitro expression and characterization of the translation start site of the psbA gene product (QB protein) from higher plants.
pubmed:publicationType
Journal Article