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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-10-19
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pubmed:abstractText |
In this report we demonstrate that ovine and bovine luteal cells synthesise oxytocin by way of a precursor protein similar to that found in the hypothalamus. Isolated ovine or bovine luteal cells were incubated for up to 12 h with [35S]cysteine. Neurophysin-Sepharose column separation and HPLC of cell extracts demonstrated the presence of [35S]oxytocin. Incorporation of [35S]cysteine was confirmed by performic acid oxidation. Immunoprecipitation of cell extract with anti-rat oxytocin-neurophysin followed by SDS-PAGE yielded 2 radioactive bands of 14 kDa and 11-12 kDa. Immunoprecipitation with anti-oxytocin yielded 1 band at 14 kDa. On SDS-PAGE the 14 kDa band had a similar mobility to rat-hypothalamic oxytocin precursor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-5793
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
262-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6381099-Animals,
pubmed-meshheading:6381099-Cattle,
pubmed-meshheading:6381099-Chromatography, High Pressure Liquid,
pubmed-meshheading:6381099-Corpus Luteum,
pubmed-meshheading:6381099-Cysteine,
pubmed-meshheading:6381099-Female,
pubmed-meshheading:6381099-Formic Acids,
pubmed-meshheading:6381099-Immunosorbent Techniques,
pubmed-meshheading:6381099-Luteal Cells,
pubmed-meshheading:6381099-Oxidation-Reduction,
pubmed-meshheading:6381099-Oxytocin,
pubmed-meshheading:6381099-Protein Precursors,
pubmed-meshheading:6381099-Sheep,
pubmed-meshheading:6381099-Sulfur Radioisotopes
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pubmed:year |
1984
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pubmed:articleTitle |
Biosynthesis of oxytocin in the corpus luteum.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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