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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1984-7-17
|
| pubmed:abstractText |
Most of the isolates of Bordetella bronchiseptica obtained by this laboratory possessed a characteristic colonial morphology when grown on Bordet- Gengou agar (BGA) at 37 degrees C. The colonies appeared domed (Dom+) with a smooth colonial surface (Scs+) and a clear zone of hemolysis ( Hly +). From these Dom+ Scs+ Hly + BGA colony types arose flat (Dom-), smooth colonial surface (Scs+) and nonhemolytic ( Hly -) variants at frequencies of 10(-2) to 10(-3). Isogenic pairs of Dom+ Scs+ Hly + and Dom- Scs+ Hly - BGA phenotype variants (BGA-PVs) were picked from 11 strains of B. bronchiseptica, and their whole cell lysates were compared with each other by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Characteristic SDS-PAGE profiles were observed for each of the Dom+ Scs+ Hly + and Dom- Scs+ Hly - BGA-PVs with regard to (i) surface-exposed proteins, based on autoradiographs of 125I- Iodogen -labeled organisms, (ii) polypeptide differences, based on gels stained with Coomassie brilliant blue R-250, and (iii) lipopolysaccharide differences based on gels stained with silver after oxidation with periodic acid. SDS-PAGE profiles were then used to monitor the phenotypes expressed by Dom+ Scs+ Hly + and Dom- Scs+ Hly - BGA-PVs transferred and grown on brucella agar, Trypticase soy agar, and nutrient agar. When grown on non-BGA media, the Dom+ Scs+ Hly + BGA-PVs from six of eight strains showed SDS-PAGE profiles identical to those of Dom- Scs+ Hly - BGA-PVs. This phenotypic change was reversible even after 15 subcultures on the non-BGA media, since Dom+ Scs+ Hly + organisms passed back onto BGA expressed both Dom+ Scs+ Hly + colonial morphology and Dom+ Scs+ Hly + SDS-PAGE profiles. The influence of cultural conditions on maintenance of virulence is discussed.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-415006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-504806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-6176137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-6187729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-6247626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-6263983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-6279517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-6317567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-6830009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6373614-858784
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0019-9567
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
681-7
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:6373614-Animals,
pubmed-meshheading:6373614-Bacterial Proteins,
pubmed-meshheading:6373614-Bacteriological Techniques,
pubmed-meshheading:6373614-Bordetella,
pubmed-meshheading:6373614-Culture Media,
pubmed-meshheading:6373614-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6373614-Guinea Pigs,
pubmed-meshheading:6373614-Phenotype
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Phenotypic variation and modulation in Bordetella bronchiseptica.
|
| pubmed:publicationType |
Journal Article
|