Linked Life Data

6370741

Source:http://linkedlifedata.com/resource/pubmed/id/6370741

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-6-7
pubmed:abstractText
The possible sites of 5.8S:25S rRNA interaction in Saccharomyces cerevisiae are investigated by blot-hybridization of in vivo 32P-labelled 5.8S rRNA with restriction fragments from the 25S rRNA gene. Strong hybridization signals are obtained with fragments from the 5'-end (nucleotides 1 to 494) and the 3'-end (3066 to 3391) of the gene. The fragments from the remaining part of the gene are negative. A computer analysis of the known Saccharomyces cerevisiae 5.8S (Rubin 1973) and 25S (Georgiev et al. 1981) rRNA sequences show a markedly higher complementarity between 5.8S rRNA and the 5'- and 3'-terminal segments of 25S rRNA corresponding to the hybridization positive rDNA fragments. In accordance with the experimental and sequence analysis data, two alternative end-to-end base pairing models of possible 5.8S:25S rRNA binding are proposed. Both models imply that 5.8S rRNA connects the 5'- and 3'-terminal segments of 25S rRNA, but differ in the extent of preservation of the secondary structure typical of free 5.8S rRNA. It is suggested that the requirement for 5'- and 3'-end binding of 23S rRNA in Escherichia coli is conserved in evolution and that in eukaryotes 5.8S rRNA plays the role of joining together the two ends of L-rRNA molecules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0015-5500
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-14
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Evidence for interaction of 5.8S rRNA with the 5'- and 3'- terminal segments of Saccharomyces cerevisiae 25S rRNA.
pubmed:publicationType
Journal Article