Linked Life Data

6366512

Source:http://linkedlifedata.com/resource/pubmed/id/6366512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-3-29
pubmed:abstractText
A functional SNF1 gene product is required to derepress expression of many glucose-repressible genes in Saccharomyces cerevisiae. Strains carrying a snf1 mutation are unable to grow on sucrose, galactose, maltose, melibiose, or nonfermentable carbon sources; utilization of these carbon sources is regulated by glucose repression. The inability of snf1 mutants to utilize sucrose results from failure to derepress expression of the structural gene for invertase at the RNA level. We isolated recombinant plasmids carrying the SNF1 gene by complementation of the snf1 defect in S. cerevisiae. A 3.5-kilobase region is common to the DNA segments cloned in five different plasmids. Transformation of S. cerevisiae with an integrating vector carrying a segment of the cloned DNA resulted in integration of the plasmid at the SNF1 locus. This result indicates that the cloned DNA is homologous to sequences at the SNF1 locus. By mapping a plasmid marker linked to SNF1 in this transformant, we showed that the SNF1 gene is located on chromosome IV. We then mapped snf1 to a position 5.6 centimorgans distal to rna3 on the right arm; snf1 is not extremely closely linked to any previously mapped mutation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-17247336, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-237205, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-347451, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-395030, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-6246361, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-6273866, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-6341817, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-6366513, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-6749597, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-7010111, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-7039847, http://linkedlifedata.com/resource/pubmed/commentcorrection/6366512-7040163
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't