Linked Life Data

6366060

Source:http://linkedlifedata.com/resource/pubmed/id/6366060

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-3-30
pubmed:abstractText
The existing methods for phagocytosis evaluation are inadequate for assessing all the real events occurring during phagocytosis, or for continuously following the kinetics of the process. Our purpose is to establish the use of luminous bacteria as an object for phagocytosis. The bioluminescence test offers an easy and simple method to determine the kinetics of phagocytosis by following the luminescence of the bacteria. The terrestrial luminous bacteria Vibrio cholerae var. albensis are readily phagocytosed by polymorphonuclear (PMN) cells. The correlation coefficient between the decrease in luminescence and the decrease in viable count is 0.999. The rate of decrease in luminescence and the residual level of luminescence after 60 min of phagocytosis are proportional to the rate of increase in phagocytosis induced chemiluminescence, and to its maximal level, respectively. Opsonization requirements are comparable in both tests. Different inhibitors of the phagocytosis process caused similar changes in the rates of the bio- and chemiluminescence (correlation coefficient 0.974), and in the luminescence maximal level (correlation coefficient 0.804). The validity of the bioluminescence assay being proved, it is suggested as an alternative assay for phagocytosis assessment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-63
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
The use of luminous bacteria for determination of phagocytosis.
pubmed:publicationType
Journal Article