Linked Life Data

6363205

Source:http://linkedlifedata.com/resource/pubmed/id/6363205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1984-3-5
pubmed:abstractText
Some originally haploid strains of yeast Peterhof breeding stocks obtained in tetrad analysis of diploids turned out to be spontaneous autodiploids after storage. They produced polyploid hybrids, as shown by tetrad analysis. An express-test for discrimination between haploids and autodiploids, based on the frequency of mitotic homozygotisation for a recessive marker has been developed. It is 10-100 times higher in heterozygous diploids than in duplex triploids. Tester strains bearing selective recessive lys2, sup1 or sup2 marker have been obtained. The hybrids of the tester strains and those to be tested were plated on media selective for mitotic segregants homozygous for lys2 (medium with alpha-aminoadipic acid and lysine) or sup1, sup2 (medium without adenine, while the hybrid was homozygous for ochre nonsense ade1-6 suppressible by either sup1 or sup2); UV-induced mitotic homozygotisation was scored. By our method, we have shown that spontaneous autodiploids constitute 70% of strains under investigation in Peterhof breeding stocks.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0016-6758
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1934-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
[Genetic express-test for distinguishing between haploids and autodiploids in the yeast Saccharomyces].
pubmed:publicationType
Journal Article, English Abstract