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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1984-3-5
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pubmed:abstractText |
A myeloid cell line, designated PL-21, was established from the peripheral blood of a patient with acute promyelocytic leukemia. The PL-21 cell line grew in single-cell suspension, with a doubling time of 48-64 hr, and consisted of promyelocytes with fine immature nuclei and prominent azurophilic granules in the cytoplasm. PL-21 cells were positive for peroxidase, naphthol AS-D chloroacetate esterase, and Sudan Black B staining. Under the usual culture conditions, a small proportion of these cells differentiated into mature granulocytes, and this differentiation was enhanced by the addition of dimethyl sulfoxide in the culture medium. PL-21 cells had receptors for the Fc portion of IgG and complement, intracytoplasmic lysozyme and phagocytic activity, but lacked Epstein-Barr virus-associated nuclear antigen. Chromosome analysis of this cell line revealed a human male polyploid karyotype with 13q+ and double minute chromosomes. This new myeloid cell line may provide useful material for the study of proliferation and differentiation of human leukemia cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
254-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:6362749-Adult,
pubmed-meshheading:6362749-Cell Differentiation,
pubmed-meshheading:6362749-Cell Line,
pubmed-meshheading:6362749-Cells, Cultured,
pubmed-meshheading:6362749-Dimethyl Sulfoxide,
pubmed-meshheading:6362749-Humans,
pubmed-meshheading:6362749-Immunoenzyme Techniques,
pubmed-meshheading:6362749-Karyotyping,
pubmed-meshheading:6362749-Leukemia, Myeloid, Acute,
pubmed-meshheading:6362749-Leukocytes,
pubmed-meshheading:6362749-Male,
pubmed-meshheading:6362749-Mediastinal Neoplasms,
pubmed-meshheading:6362749-Muramidase,
pubmed-meshheading:6362749-Phagocytosis,
pubmed-meshheading:6362749-Rosette Formation
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pubmed:year |
1984
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pubmed:articleTitle |
Establishment of a new peroxidase-positive human myeloid cell line, PL-21.
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pubmed:publicationType |
Journal Article,
Case Reports
|