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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1984-1-7
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pubmed:abstractText |
The cellular tumor antigen p53 was isolated from mouse neuroblastoma cells and was found in a form not complexed to another protein. The p53 in these cells was stable, turning over about every 10 h. Its methionine-labelled tryptic peptides were very similar to those of the p53 isolated from SV40-transformed mouse cells. The labelled protein was purified from neuroblastoma cells by immunoaffinity using specific monoclonal antibodies and was about 80% radiochemically pure. Furthermore, the purified p53 sedimented in sucrose gradients with a sedimentation coefficient of approximately 8S. This correlated with the sedimentation coefficient of p53 prior to purification, showing that the purified protein retained its native size.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0020-7136
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
597-602
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:6358054-Animals,
pubmed-meshheading:6358054-Antibodies, Monoclonal,
pubmed-meshheading:6358054-Antigens, Neoplasm,
pubmed-meshheading:6358054-Cell Line,
pubmed-meshheading:6358054-Centrifugation, Density Gradient,
pubmed-meshheading:6358054-Chromatography, Affinity,
pubmed-meshheading:6358054-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6358054-Half-Life,
pubmed-meshheading:6358054-Mice,
pubmed-meshheading:6358054-Neoplasm Proteins,
pubmed-meshheading:6358054-Neuroblastoma,
pubmed-meshheading:6358054-Phosphoproteins,
pubmed-meshheading:6358054-Tumor Suppressor Protein p53,
pubmed-meshheading:6358054-Tumor Virus Infections
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pubmed:year |
1983
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pubmed:articleTitle |
Immunoaffinity chromatography of a cellular tumor antigen from mouse neuroblastoma cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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