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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1983-12-20
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pubmed:abstractText |
By cloning a 3.6-kb EcoRI fragment of the Escherichia coli chromosome with pBR322 we located more precisely recF relative to dnaN. By deletion mapping we localized functional recF to a 1.65-kb region of the cloned fragment and allowed rough mapping of the C terminus of dnaN. Cloned recF+, separated from functional flanking genes dnaN and gyrB, complemented chromosomal recF mutations presumably by coding for a cytodiffusible product. The protein encoded by dnaN was observed as a band on a polyacrylamide gel from minicells. Identification of a recF protein was not made.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0147-619X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
101-10
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6356186-Bacterial Proteins,
pubmed-meshheading:6356186-Chromosome Deletion,
pubmed-meshheading:6356186-Chromosome Mapping,
pubmed-meshheading:6356186-Cloning, Molecular,
pubmed-meshheading:6356186-DNA, Bacterial,
pubmed-meshheading:6356186-Escherichia coli,
pubmed-meshheading:6356186-Genes, Bacterial,
pubmed-meshheading:6356186-Mutation,
pubmed-meshheading:6356186-Plasmids
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pubmed:year |
1983
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pubmed:articleTitle |
Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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