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pubmed:abstractText |
Chemical modification was used to study the conformational changes occurring in yeast tRNAPhe after the Y-base excision. The chemical probe was the adenine- and cytosine-specific reagent chloroacetaldehyde. Comparison of the modification patterns in tRNAPhe and tRNAPhe-Y shows that seven bases, adenines 35, 36 and 38 in the anticodon loop and adenines 73, 76 and cytosines 74, 75 in the 3'-terminus were modified in both tRNAs with a quantitative difference in the modification level of the anticodon loop bases. The most interesting, however, is the qualitative difference consisting in modification of cytosine-60 in the T psi C loop of tRNAPhe-Y. Some aspects of the mechanism of this long-distance conformational transition are briefly discussed.
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