Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1983-12-17
|
| pubmed:abstractText |
Lymphoid precursor cells are present in long-term bone marrow cultures (LTBMC), but their differentiation into mature lymphocytes is blocked. A quantitative assay for B cell precursors in LTBMC, which gives a linear relationship between the number of grafted LTBMC cells and the frequency of B cell colony forming units (CFU-B) in the spleen and bone marrow of immunodeficient CBA/N mice 19 days after reconstitution, is described. Characterization of the B cell precursor indicates that this assay is detecting a very early precursor and not a B lymphocyte or a late pre-B cell. This conclusion is based on the observations that a) pre-B cells transformable by Abelson murine leukemia virus are not present in LTBMC by 3 days postrecharge and CFU-B are absent by 6 days postrecharge; b) late B cell progenitors capable of rapid repopulation of irradiated CBA/N mice are not present in LTBMC, since a lag in the kinetics of B cell reconstitution in animals grafted with LTBMC cells is observed compared with fresh bone marrow cells; c) the B cell precursors in LTBMC have high proliferative potential, since they can stably repopulate recipient mice for at least 8 wk postreconstitution and through two serial passages in irradiated CBA/N recipients; and d) the B cell precursors are large, rapidly sedimenting cells as determined by velocity sedimentation. The serial transplantation experiment further shows that a split is often observed between lymphoid and myeloid reconstituting ability of LTBMC cells. The LTBMC B cell precursor may be a pluripotent stem cell or a lymphoid stem cell, although its differentiative potential remains to be determined.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
131
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2240-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6355292-Abelson murine leukemia virus,
pubmed-meshheading:6355292-Animals,
pubmed-meshheading:6355292-B-Lymphocytes,
pubmed-meshheading:6355292-Blood Sedimentation,
pubmed-meshheading:6355292-Bone Marrow,
pubmed-meshheading:6355292-Bone Marrow Cells,
pubmed-meshheading:6355292-Bone Marrow Transplantation,
pubmed-meshheading:6355292-Cell Adhesion,
pubmed-meshheading:6355292-Cell Separation,
pubmed-meshheading:6355292-Cell Transformation, Viral,
pubmed-meshheading:6355292-Cells, Cultured,
pubmed-meshheading:6355292-Colony-Forming Units Assay,
pubmed-meshheading:6355292-Female,
pubmed-meshheading:6355292-Hematopoiesis,
pubmed-meshheading:6355292-Kinetics,
pubmed-meshheading:6355292-Male,
pubmed-meshheading:6355292-Mice,
pubmed-meshheading:6355292-Mice, Inbred BALB C,
pubmed-meshheading:6355292-Mice, Inbred CBA,
pubmed-meshheading:6355292-Stem Cells,
pubmed-meshheading:6355292-Time Factors
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Characterization of early B lymphocyte precursors present in long-term bone marrow cultures.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|