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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1983-12-17
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pubmed:abstractText |
Multidrug resistance is a complex pleiotropic phenotype of cross-resistance and collateral sensitivity to unrelated compounds observed in many mammalian cell mutants selected for resistance to single agents. In Chinese hamster ovary cells, colchicine-resistant mutants expressing this phenotype have been characterized extensively. Such mutants arise apparently from a single genetic event, and the basis of this phenotype appears to be localized at the membrane level, resulting in altered drug permeability. Expression of a 170,000-dalton surface glycoprotein (P-glycoprotein) has been identified to correlate with the multidrug-resistance phenotype. Selection of a second mutation in colchicine-resistant mutants, for resistance to phytohemagglutinin, results in an alteration of the carbohydrate moiety in P-glycoprotein and other surface components. This mutation does not noticeably affect the multi-drug-resistance phenotype. The altered permeability of mutant cells to drugs, however, can be modulated by nonionic detergents or metabolic inhibitors. These findings are consistent with a molecular mechanism of multidrug resistance whereby the pleiotropic response of the cell is mediated by an overexpression of a cell-surface protein, the P-glycoprotein.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Colchicine,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0361-5960
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
869-74
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6354434-Animals,
pubmed-meshheading:6354434-Antineoplastic Agents,
pubmed-meshheading:6354434-Cell Line,
pubmed-meshheading:6354434-Colchicine,
pubmed-meshheading:6354434-Cricetinae,
pubmed-meshheading:6354434-Cricetulus,
pubmed-meshheading:6354434-Drug Resistance,
pubmed-meshheading:6354434-Female,
pubmed-meshheading:6354434-Glycoproteins,
pubmed-meshheading:6354434-Membrane Proteins,
pubmed-meshheading:6354434-Mutation,
pubmed-meshheading:6354434-Ovary,
pubmed-meshheading:6354434-Phenotype,
pubmed-meshheading:6354434-Phytohemagglutinins
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pubmed:year |
1983
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pubmed:articleTitle |
Multidrug-resistance phenotype in Chinese hamster ovary cells.
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pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't
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