Linked Life Data

6353247

Source:http://linkedlifedata.com/resource/pubmed/id/6353247

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5934
pubmed:dateCreated
1983-11-23
pubmed:abstractText
Insulin regulates the synthesis of several proteins in a variety of tissues. Before techniques were available to quantify the amount of specific mRNAs, insulin was thought to regulate the synthesis of proteins by influencing the rate of translation of a fixed amount of mRNA. A very different interpretation is called for by experiments which show that insulin alters the amount of several specific mRNAs, but little is known about the mechanism. Insulin decreases the rate of synthesis of the critical gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK) in both liver and H4IIE heptoma cells. We recently showed that insulin acts directly on H4IIE cells to decrease mRNAPEPCK activity without any other hormone intermediaries. This effect is mediated by the insulin receptor and occurs at insulin concentrations which are well within the physiological range range (10(-12)--10(-9) M). Here we extend these studies to show that insulin specifically inhibits transcription of the PEPCK gene. This inhibition results in a rapid decrease in the concentration of nuclear PEPCK transcripts which is followed, in turn, by a proportionate decline in cytoplasmic mRNAPEPCK and synthesis of the protein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:volume
305
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
549-51
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:articleTitle
Inhibition of transcription of the phosphoenolpyruvate carboxykinase gene by insulin.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.